Controlling of Downy mildew (Perenosclerospora maydis) in Pulut Maize using Biological Agents Bacillus subtilis and Pseudomonas fluorescens

Abstract

Downy mildew is the most common disease affecting maize crops in Indonesia. An alternative control being developed is the use of biological agents. This study was to determine the effectiveness of B. subtilis and P. fluorescens against downy mildew on pulut maize plants. This study uses Factorial Randomize Block Design (FRBD). the first factor being the combination of bacteria its J1 (Bacillus subtilis (5 ml/l) + Pseudomonas fluorescens (20 ml/l)), J2 (Bacillus subtilis (10 ml/l) + Pseudomonas fluorescens (35ml/l)). The second factor was the application time, its T1 (2 applications), T2 (3 applications ) The parameters observed in this study were spore identification, disease incidence, disease intensity and cob weight. The results showed that B. subtilis and P. fluorescens had no effect on downy mildew attack and yield increase with disease incidence of 90%, 10% disease intensity and yield (cob weight) of 3362.1 grams