Technology of Shoot Induction Culture Tobacco Through the Addition of Benzyl Amino Purine

Abstract

The tobacco (Nicotiana tabacum L.) has a high export value for Indonesia and one type of tobacco widely developed in Jember Regency is the Kasturi Mawar. Conventional development of tobacco seeds in tobacco cultivation has several obstacles, that is it depends on the season, requires a large area, and is susceptible to pest attacks and plant diseases. The disadvantages of conventional propagation can be overcome with shoot induction technology by adding Benzyl Amino Purine (BAP). The research was carried out to determine the response of Kasturi Mawar tobacco explants in forming shoots with the addition of BAP concentration in vitro. The study was conducted from November 2023 to March 2024. It took place in the Tissue Culture Laboratory of Jember State Polytechnic at an altitude of 89 m above sea level with a latitude of 8°09'35.1"S 113°43'27.2"E. The experimental design used was a non-factorial Completely Randomized Design (CRD) with the addition of 4 levels of BAP concentration with 5 replications. The results of the research showed that adding a BAP concentration of 3 mg/liter was the optimal concentration in inducing Kasturi Mawar tobacco shoots with shoot formation speed 10 days after inoculation (DAI), an average number of shoots of 42 shoots/explant, an average explant weight of 4.7 grams/explant at 56 DAI. However, further studies are still needed to measure the response of Kasturi Mawar tobacco explants in forming shoots on the addition of BAP concentrations of more than 4 mg/liter.